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Total Protein Serum Level of Nilem Fish (Osteochillus vittatus) Fed by Chlorella vulgaris Supplementation

ZIA, Khalida (2020) Total Protein Serum Level of Nilem Fish (Osteochillus vittatus) Fed by Chlorella vulgaris Supplementation. Skripsi thesis, Universitas Jenderal Soedirman.

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Abstract

Nilem fish (Osteochillus vittatus) was freshwater fish that was widely cultivated, but most of them have not been done intensively, so the results were not optimal. The success of Nilem fish (O. vittatus) cultivation was strongly influenced by the availability of sufficient feed, so effective and efficient feed management was needed. Conventional feed sometimes does not contain enough nutrients to meet fish feed needs, so supplementation was needed. Supplementation was needed in fish cultivation to spur fish health. One supplement that can be used in fish cultivation was C. vulgaris. Chlorella vulgaris was one of the potential microalgae as an immunostimulant because it contained high protein, vitamins, minerals, fats, polysaccharides, and other nutrients. The problem that was often faced in fish cultivation was a disease that can cause a decrease in the level of fish production, soit was necessary to detect immunity status in fish. One way to detect immune status was to measure total protein levels. The objectives of this research were (1) knowing the effect of different dosages of C. vulgaris supplementation in feed on total protein levels of Nilem fish (O. vittatus) serum; (2) getting the best dosage of C. vulgaris supplementation in feed to increase the total protein level of Nilem fish (O. vittatus) serum. The research method used was an experimental research method with a completely randomized design (CRD) with five treatments and four replications. The treatments were (P0) Nilem fish (O. vittatus) were given commercial feed without C. vulgaris supplementation; (P1-P4) Nilem fish (O. vittatus) were given supplementation of C. vulgaris 2, 3, 4, and 6 g.kg-1 of commercial feed. The variables in this research consisted of independent and dependent variables. The independent variable in this research was the various doses of C. vulgaris given to Nilem fish (O. vittatus), while the dependent variable was the change in the total protein level of Nilem fish (O. vittatus) serum. The parameter was the total protein level of Nilem fish (O. vittatus) serum. Measurement of total protein levels serum used was the biuret method with a dyasis kit and spectrophotometer. Measurement of total protein levels serum was carried out twice, before treatment (day 0) and after treatment (57th day). The results of the research data were statistically analyzed using analysis of variance (ANOVA) with an error rate of 5%, then continued with the least significant difference (LSD) test. The results showed that the different dosages of C. vulgaris supplementation in feed increased the total protein levels of Nilem fish (O. vittatus) serum (p<0.05). Supplementation dose of C. vulgaris 6 g.kg-1 commercial feed was the best dose to increase the total protein level of Nilem fish (O. vittatus) serum. At this dose showed an average result of total protein levels of 5.51 g.dL-1, which was in accordance with its normal limit, which ranged from 5-8 g.dL.-1

Item Type: Thesis (Skripsi)
Nomor Inventaris: B20015
Uncontrolled Keywords: Chlorella vulgaris, Nilem fish (Osteochillus vittatus), supplementation, total protein serum.
Subjects: F > F181 Fish culture
F > F182 Fishes
Divisions: Fakultas Biologi > S1 Biologi
Depositing User: Mrs Yayuk Khotiarsih
Date Deposited: 09 Mar 2022 04:28
Last Modified: 09 Mar 2022 04:28
URI: http://repository.unsoed.ac.id/id/eprint/14183

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