BIANI, Puti (2024) Multiplication of Anthurium Plowmanii (Croat.) Microshoots on Several Media Formulations and Phytagel Concentrations. Skripsi thesis, Universitas Jenderal Soedirman.
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Abstract
Anthurium plowmanii (Croat.) is a leaf ornamental plant with considerable economic value. The propagation A. plowmanii has been carried out conventionally using seeds and separation of tillers, which result in both slow and non-uniform plants. To address the growing demand for a large quantity of good quality and uniform A. plowmanii seeds, in vitro culture technique offers a promising solution. The success of A. plowmanii multiplication in vitro is determined by several factors, including media formulation and solidifying agent. This research has been conducted with a view to study the effect of the interaction between media formulations and phytagel concentrations on A. plowmanii microshoots multiplication; as well as to determine the best media formulation and phytagel concentration for A. plowmanii microshoots multiplication. This research has been carried out experimentally using a Completely Randomized Design (CRD) on a factorial treatment pattern consisting of two factors. The first factor was the media formulation which consists of 4 levels, namely MS full strength, MS half strength, MS half strength supplemented with AB-Mix, and AB-Mix. The second factor was the concentration of phytagel which consisted of 3 levels, i.e. 2.5 g. L-1, 3 g.L-1 and 3.5 g. L-1. Each treatment combination was repeated 6 times, which resulted in 72 experimental units. The independent variables tested involved media formulations and phytagel concentrations, whereas the dependent variable observed was the growth of A. plowmanii microshoots. The results showed that the interaction between media formulations and phytagel concentrations did not affect A. plowmanii microshoot growth. However, the media formulation used controlled the shoot elongation and root formation. The most cost-effective media formulation to induce A. plowmanii microshoots multiplication was half-strength MS solidified with 2.5 g.L-1 phytagel.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Nomor Inventaris: | B24010 |
| Uncontrolled Keywords: | AB-Mix, Anthurium plowmanii Croat, Microshoots, Multiplication, Murashige & Skoog |
| Subjects: | B > B167 Biology C > C1003 Cultivated plants Flower gardening Plants |
| Divisions: | Fakultas Biologi > S1 Biologi |
| Depositing User: | Mrs Puti Biani |
| Date Deposited: | 24 Jan 2024 02:12 |
| Last Modified: | 24 Jan 2024 02:12 |
| URI: | http://repository.unsoed.ac.id/id/eprint/25297 |
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